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Exosome secreted by human gingival fibroblasts in radiation therapy inhibits osteogenic differentiation of bone mesenchymal stem cells by transferring miR-23a.
- Source :
-
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2020 Nov; Vol. 131, pp. 110672. Date of Electronic Publication: 2020 Sep 02. - Publication Year :
- 2020
-
Abstract
- Radiation-induced fibrosis is recently established as a main reason for osteoradionecrosis of the jaw (ORNJ), anti-eradiation fibrosis drugs achieve satisfactory therapeutic effects. However, the molecular mechanism remain to be fully elucidated. In this study, we found the inhibitory effect of irradiation activated gingival fibroblasts on osteogenic differentiation of human bone mesenchymal stem cells (hBMSCs). Moreover, irradiation-activated-fibroblasts significantly increased miR‑23a expression in hBMSCs. Decreased miR‑23a enhanced osteogenic differentiation of BMSCs, and elevated miR‑23a inhibited this process via directly targeting CXCL12. Finally, exosome released from irradiation-activated-fibroblasts inhibited osteogenic differentiation of BMSCs, and these exosome mediated delivery of miR-23a and further regulated miR-23a/CXCL12 axis in hBMSCs. Therefore, our findings suggest that by transferring miR-23a, exosome secreted by human gingival fibroblasts in radiation therapy serves a vital role in osteogenic differentiation of hBMSCs, which may provide novel clinical treatments for ORNJ.<br /> (Copyright © 2020 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Subjects :
- Cell Differentiation physiology
Cells, Cultured
Exosomes metabolism
Fibroblasts metabolism
Fibroblasts radiation effects
Gingiva cytology
Gingiva metabolism
Humans
Mesenchymal Stem Cells metabolism
MicroRNAs genetics
Osteogenesis physiology
Osteoradionecrosis genetics
Osteoradionecrosis metabolism
Osteoradionecrosis therapy
X-Rays adverse effects
Cell Differentiation radiation effects
Exosomes radiation effects
Gingiva radiation effects
Mesenchymal Stem Cells radiation effects
MicroRNAs biosynthesis
Osteogenesis radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 1950-6007
- Volume :
- 131
- Database :
- MEDLINE
- Journal :
- Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
- Publication Type :
- Academic Journal
- Accession number :
- 32889404
- Full Text :
- https://doi.org/10.1016/j.biopha.2020.110672