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Transcriptomic Analysis of Streptococcus suis in Response to Ferrous Iron and Cobalt Toxicity.
- Source :
-
Genes [Genes (Basel)] 2020 Sep 02; Vol. 11 (9). Date of Electronic Publication: 2020 Sep 02. - Publication Year :
- 2020
-
Abstract
- Streptococcus suis is a zoonotic pathogen causing serious infections in swine and humans. Although metals are essential for life, excess amounts of metals are toxic to bacteria. Transcriptome-level data of the mechanisms for resistance to metal toxicity in S. suis are available for no metals other than zinc. Herein, we explored the transcriptome-level changes in S. suis in response to ferrous iron and cobalt toxicity by RNA sequencing. Many genes were differentially expressed in the presence of excess ferrous iron and cobalt. Most genes in response to cobalt toxicity showed the same expression trends as those in response to ferrous iron toxicity. qRT-PCR analysis of the selected genes confirmed the accuracy of RNA sequencing results. Bioinformatic analysis of the differentially expressed genes indicated that ferrous iron and cobalt have similar effects on the cellular processes of S. suis . Ferrous iron treatment resulted in down-regulation of several oxidative stress tolerance-related genes and up-regulation of the genes in an amino acid ABC transporter operon. Expression of several genes in the arginine deiminase system was down-regulated after ferrous iron and cobalt treatment. Collectively, our results suggested that S. suis alters the expression of multiple genes to respond to ferrous iron and cobalt toxicity.
- Subjects :
- ATP-Binding Cassette Transporters genetics
Animals
Bacterial Proteins genetics
Down-Regulation genetics
Gene Expression Profiling methods
Gene Expression Regulation, Bacterial genetics
Hydrolases genetics
Operon genetics
Oxidative Stress genetics
Swine
Cobalt toxicity
Iron toxicity
Streptococcus suis genetics
Transcriptome genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2073-4425
- Volume :
- 11
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Genes
- Publication Type :
- Academic Journal
- Accession number :
- 32887434
- Full Text :
- https://doi.org/10.3390/genes11091035