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Genotypic and phenotypic characterisation of clinical isolates of methicillin-resistant Staphylococcus aureus in two different geographical locations of Iran.
- Source :
-
Indian journal of medical microbiology [Indian J Med Microbiol] 2020 Apr-Jun; Vol. 38 (2), pp. 162-168. - Publication Year :
- 2020
-
Abstract
- Background: Methicillin-resistant Staphylococcus aureus (MRSA) has become more prevalent all over the world and it is important to determine MRSA prevalence and typing in different regions. The present study was carried out to determine the prevalence and frequency of circulating molecular types of MRSA isolates as well as their antibiotics susceptibility in Tabriz and Kerman cities of Iran.<br />Materials and Methods: A total of 230 S. aureus isolates were collected from Tabriz (n=125) and Kerman (n=105) during January to December 2018. MRSA isolates were identified by PCR amplification of nuc and mec A genes. Antibiotic susceptibility of MRSA isolates were determined by Kirby-Bauer disk diffusion method. Multiplex PCR was exploited to detect various types of SCCmec.<br />Results: The MRSA prevalence was 51/125 (40.8%) in Tabriz and 60/105 (57.1%) in Kerman. Overall, 36/51 (70.58%) and 15/51 (29.41%) isolates and 37/60 (61.66%) and 23/60 (38.34%) isolates were isolated from inpatients and outpatients in Tabriz and Kerman, respectively. Almost all of the isolates were resistant to penicillin and all of them were sensitive to linezolid. Thirty five (68.2%) and 34(56.6%) of MRSA isolates in Tabriz and Kerman were determined as MDR, respectively. SCCmec typing showed that the frequent SCCmec type in both Tabriz and Kerman cities was SCCmec III (56.86% and 55%, respectively).<br />Conclusion: The high prevalence of MRSA makes it necessary to revisit the antibiotics administration by physicians. Indeed, periodic evaluation of antibacterial susceptibility patterns of the MRSA strains is required for efficient treatment of MRSA infections.<br />Competing Interests: None
- Subjects :
- Adolescent
Adult
Aged
Bacterial Proteins genetics
Child
Child, Preschool
Disk Diffusion Antimicrobial Tests
Genes, Bacterial
Humans
Interspersed Repetitive Sequences
Iran epidemiology
Linezolid pharmacology
Methicillin-Resistant Staphylococcus aureus isolation & purification
Micrococcal Nuclease genetics
Middle Aged
Molecular Typing
Multiplex Polymerase Chain Reaction
Penicillin Resistance
Penicillin-Binding Proteins genetics
Staphylococcal Infections epidemiology
Anti-Bacterial Agents pharmacology
Methicillin-Resistant Staphylococcus aureus drug effects
Methicillin-Resistant Staphylococcus aureus genetics
Staphylococcal Infections microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1998-3646
- Volume :
- 38
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Indian journal of medical microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 32883929
- Full Text :
- https://doi.org/10.4103/ijmm.IJMM_20_153