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Cross protection by inactivated recombinant influenza viruses containing chimeric hemagglutinin conjugates with a conserved neuraminidase or M2 ectodomain epitope.
- Source :
-
Virology [Virology] 2020 Nov; Vol. 550, pp. 51-60. Date of Electronic Publication: 2020 Aug 22. - Publication Year :
- 2020
-
Abstract
- Influenza virus neuraminidase (NA) contains a universally conserved epitope (NAe, NA <subscript>222-230</subscript> ). However, no studies have reported vaccines targeting this NA conserved epitope and inducing antibodies recognizing NAe. The extracellular domain of M2 (M2e) is considered as an attractive target for a universal influenza vaccine. We generated recombinant influenza H1N1 viruses expressing conserved epitopes in hemagglutinin (HA) molecules: NAe (NAe-HA) or M2e (M2e-HA) within the HA head domain. Inactivated recombinant NAe-HA and M2e-HA viruses were more effective in inducing IgG antibodies specific for an inserted conserved epitope than live recombinant virus. Recombinant inactivated M2e-HA virus vaccination induced cross protection against H3N2 virus with less weight loss compared to NAe-HA and was more effective in inducing humoral and cellular M2e immune responses. This study provides insight into developing recombinant influenza virus vaccines compatible with current platforms to induce antibody responses to conserved poorly immunogenic epitopes.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Antibodies, Viral biosynthesis
Cross Protection
Epitopes chemistry
Epitopes immunology
Female
Hemagglutinin Glycoproteins, Influenza Virus genetics
Humans
Immunity, Cellular drug effects
Immunity, Humoral drug effects
Influenza A Virus, H1N1 Subtype drug effects
Influenza A Virus, H1N1 Subtype genetics
Influenza A Virus, H3N2 Subtype drug effects
Influenza A Virus, H3N2 Subtype genetics
Influenza A Virus, H3N2 Subtype immunology
Influenza A Virus, H5N1 Subtype drug effects
Influenza A Virus, H5N1 Subtype genetics
Influenza A Virus, H5N1 Subtype immunology
Influenza A Virus, H9N2 Subtype drug effects
Influenza A Virus, H9N2 Subtype genetics
Influenza A Virus, H9N2 Subtype immunology
Influenza Vaccines administration & dosage
Influenza Vaccines genetics
Mice
Mice, Inbred BALB C
Neuraminidase genetics
Orthomyxoviridae Infections immunology
Orthomyxoviridae Infections virology
Reassortant Viruses drug effects
Reassortant Viruses genetics
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins immunology
Vaccination methods
Vaccines, Inactivated
Viral Matrix Proteins genetics
Hemagglutinin Glycoproteins, Influenza Virus immunology
Influenza A Virus, H1N1 Subtype immunology
Influenza Vaccines biosynthesis
Neuraminidase immunology
Orthomyxoviridae Infections prevention & control
Reassortant Viruses immunology
Viral Matrix Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0341
- Volume :
- 550
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 32882637
- Full Text :
- https://doi.org/10.1016/j.virol.2020.08.003