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Drug-induced reactive oxygen species-mediated inhibitory effect on growth of Trypanosoma evansi in axenic culture system.
- Source :
-
Parasitology research [Parasitol Res] 2020 Oct; Vol. 119 (10), pp. 3481-3489. Date of Electronic Publication: 2020 Sep 01. - Publication Year :
- 2020
-
Abstract
- Trypanosoma evansi, an extracellular haemoflagellate, has a wide range of hosts receptive and susceptible to infection, in which it revealed highly inconsistent clinical effects. Drugs used for the treatment of trypanosomosis have been utilized for more than five decades and have several problems like local and systemic toxicity. In the present investigation, imatinib and sorafenib were selected as drugs as they are reported to have the potential to cause reactive oxygen species (ROS)-mediated effect in cancer cells. Both have also been reported to have potential against T. brucei, T. cruzi and Leishmania donovani. To date, imatinib and sorafenib have not evaluated for their growth inhibitory effect against T. evansi. Imatinib and sorafenib showed significant (p < 0.001) inhibition on parasite growth and multiplication with IC <subscript>50</subscript> (50% inhibitory concentration) values 6.12 μM and 0.33 μM respectively against T. evansi. Both the drug molecules demonstrated for the generation of ROS in T. evansi and were found up to 65% increased level of ROS as compared with negative control in the axenic culture system. Furthermore, different concentrations of imatinib and sorafenib were found non-toxic on horse peripheral blood mononuclear cells and Vero cell lines. Also, in conclusion, our results demonstrated that imatinib- and sorafenib-induced generation of ROS contributed inhibitory effect on the growth of Trypanosoma evansi in an axenic culture system.
- Subjects :
- Animals
Axenic Culture
Cell Survival drug effects
Cells, Cultured
Chlorocebus aethiops
Horses
Imatinib Mesylate pharmacology
Inhibitory Concentration 50
Leukocytes, Mononuclear drug effects
Sorafenib pharmacology
Trypanosoma metabolism
Vero Cells
Reactive Oxygen Species metabolism
Trypanocidal Agents pharmacology
Trypanosoma drug effects
Trypanosoma growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1432-1955
- Volume :
- 119
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Parasitology research
- Publication Type :
- Academic Journal
- Accession number :
- 32869169
- Full Text :
- https://doi.org/10.1007/s00436-020-06861-7