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The Effects of Proton Pump Inhibitors (Pantoprazole) on Pentylenetetrazole-Induced Epileptic Seizures in Rats and Neurotoxicity in the SH-SY5Y Human Neuroblastoma Cell Line.
- Source :
-
Cellular and molecular neurobiology [Cell Mol Neurobiol] 2021 Jan; Vol. 41 (1), pp. 173-183. Date of Electronic Publication: 2020 Aug 29. - Publication Year :
- 2021
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Abstract
- Recent studies have shown that proton pump inhibitors have positive effects on the nervous system. However, its effect on epileptic seizure and neuronal damage are still unclear. In this study, it was aimed to investigate the effect of pantoprazole on pentylenetetrazole-induced epileptic seizures in rats and neurotoxicity in the SH-SY5Y cell line. Animals were divided into three groups: control, saline (1 mL/kg serum physiologic), and pantoprazole (10 mg/kg). Pentylenetetrazole (45 mg/kg) was given to induce a seizure and a passive avoidance test trial was carried out to evaluate memory function. 8-hydroxy-2'-deoxyguanosine (8-OHdG), caspase-3, and brain-derived neurotrophic factor (BDNF) levels were measured in the brain by commercial kits. SH-SY5Y cells were treated with saline or pantoprazole for one hour, and then pentylenetetrazole (30 µm) was added to the medium to induce neurotoxicity. After 24 h, cell viability, total antioxidant, total oxidant status, and apoptosis were measured in SH-SY5Y cells. It was found that pantoprazole treatment postponed epileptic seizure onset, protected memory, reduced 8-OHdG, caspase-3, and also increased BDNF in the brain. In addition, it blocked pentylenetetrazole toxicity, apoptosis, increased antioxidant, and decreased oxidant status in SH-SY5Y cells. Pantoprazole significantly improved seizure, oxidative stress, and apoptosis. Thus, pantoprazole could be used as a supportive therapeutic agent in epilepsy.
- Subjects :
- 8-Hydroxy-2'-Deoxyguanosine metabolism
Animals
Antioxidants metabolism
Apoptosis drug effects
Brain pathology
Brain-Derived Neurotrophic Factor metabolism
Caspase 3 metabolism
Cell Line, Tumor
Cell Survival drug effects
Humans
Male
Oxidants metabolism
Oxidative Stress drug effects
Pantoprazole pharmacology
Pentylenetetrazole
Proton Pump Inhibitors pharmacology
Rats, Wistar
Seizures chemically induced
Seizures pathology
Rats
Neuroblastoma pathology
Neurotoxins toxicity
Pantoprazole therapeutic use
Proton Pump Inhibitors therapeutic use
Seizures drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6830
- Volume :
- 41
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cellular and molecular neurobiology
- Publication Type :
- Academic Journal
- Accession number :
- 32862257
- Full Text :
- https://doi.org/10.1007/s10571-020-00956-6