Enzymatic Building-Block Synthesis for Solid-Phase Automated Glycan Assembly.

Authors :: Marchesi A
Parmeggiani F
Louçano J
Mattey AP
Huang K
Gupta T
Salwiczek M
Flitsch SL
Source :: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2020 Dec 07; Vol. 59 (50), pp. 22456-22459. Date of Electronic Publication: 2020 Oct 02.
Publication Year :: 2020
Abstract: Automated chemical oligosaccharide synthesis is an attractive concept that has been successfully applied to a large number of target structures, but requires excess quantities of suitably protected and activated building blocks. Herein we demonstrate the use of biocatalysis to supply such reagents for automated synthesis. By using the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis we demonstrate fast and robust access to the LacNAc motif, common to many cell-surface glycans, starting from either lactose or sucrose as glycosyl donors. The enzymatic product was shown to be successfully incorporated as a complete unit into a tetrasaccharide target by automated assembly.<br /> (© 2020 The Authors. Published by Wiley-VCH GmbH.)