Bradyrhizobium campsiandrae sp. nov., a nitrogen-fixing bacterial strain isolated from a native leguminous tree from the Amazon adapted to flooded conditions.

The nitrogen-fixing bacterial strain UFLA 01-1174 ^T was isolated from nodules of Campsiandra laurilifolia Benth. originating from the Amazon region, Brazil. Its taxonomic position was defined using a polyphasic approach. Analysis of the 16S rRNA gene placed the strain in the Bradyrhizobium genus, the closest species being B. guangdongense CCBAU 51649 ^T and B. guangzhouense CCBAU 51670 ^T , both with 99.8% similarity. Multilocus sequence analysis (MLSA) of recA, gyrB, glnII, rpoB, atpD, and dnaK indicated that UFLA 01-1174 ^T  is a new species, most closely related to B. stylosanthis BR 446 ^T (94.4%) and B. manausense BR 3351 ^T (93.7%). Average nucleotide identity (ANI) differentiated UFLA 01-1174 ^T from the closest species with values lower than 90%. The G + C content in the DNA of UFLA 01-1174 ^T  is 63.6 mol%. Based on this data, we conclude that the strain represents a new species. The name proposed is Bradyrhizobium campsiandrae, with UFLA 01-1174 ^T (= INPA 394B ^T  = LMG 10099 ^T ) as type strain.