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Identification of candidate microRNAs from Ostreid herpesvirus-1 (OsHV-1) and their potential role in the infection of Pacific oysters (Crassostrea gigas).
- Source :
-
Molecular immunology [Mol Immunol] 2020 Oct; Vol. 126, pp. 153-164. Date of Electronic Publication: 2020 Aug 24. - Publication Year :
- 2020
-
Abstract
- Oyster production is an economic activity of great interest worldwide. Recently, oysters have been suffering significant mortalities from OsHV-1infection, which has resulted in substantial economic loses in several countries around the world. Understanding viral pathogenicity mechanisms is of central importance for the establishment of disease control measures. Thus, the present work aimed to identify and characterize miRNAs from OsHV-1 as well as to predict their target transcripts in the virus and the host. OsHV-1 genome was used for the in silico discovery of pre-miRNAs. Subsequently, viral and host target transcripts of the OsHV-1 miRNAs were predicted according to the base pairing interaction between mature miRNAs and mRNA 3' untranslated regions (UTRs). Six unique pre-miRNAs were found in different regions of the viral genome, ranging in length from 85 to 172 nucleotides. A complex network of self-regulation of viral gene expression mediated by the miRNAs was identified. These sequences also seem to have a broad ability to regulate the expression of host immune-related genes, especially those associated with pathogen recognition. Our results suggest that OsHV-1 encodes miRNAs with important functions in the infection process, inducing self-regulation of viral transcripts, as well as affecting the regulation of Pacific oyster transcripts related to immunity. Understanding the molecular basis of host-pathogen interactions can help mitigate the recurrent events of oyster mass mortalities by OsHV-1 observed worldwide.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Aquaculture
Computational Biology
Crassostrea genetics
Crassostrea immunology
DNA Viruses genetics
DNA Viruses immunology
Gene Expression Regulation immunology
Gene Regulatory Networks genetics
Gene Regulatory Networks immunology
Host-Pathogen Interactions immunology
MicroRNAs genetics
MicroRNAs isolation & purification
RNA, Viral genetics
RNA, Viral isolation & purification
Crassostrea virology
DNA Viruses pathogenicity
Host-Pathogen Interactions genetics
MicroRNAs metabolism
RNA, Viral metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1872-9142
- Volume :
- 126
- Database :
- MEDLINE
- Journal :
- Molecular immunology
- Publication Type :
- Academic Journal
- Accession number :
- 32853878
- Full Text :
- https://doi.org/10.1016/j.molimm.2020.08.004