Warmer, faster, stronger: Ca 2+ cycling in avian myocardium.

Birds occupy a unique position in the evolution of cardiac design. Their hearts are capable of cardiac performance on par with, or exceeding that of mammals, and yet the structure of their cardiomyocytes resembles those of reptiles. It has been suggested that birds use intracellular Ca ²⁺ stored within the sarcoplasmic reticulum (SR) to power contractile function, but neither SR Ca ²⁺ content nor the cross-talk between channels underlying Ca ²⁺ -induced Ca ²⁺ release (CICR) have been studied in adult birds. Here we used voltage clamp to investigate the Ca ²⁺ storage and refilling capacities of the SR and the degree of trans-sarcolemmal and intracellular Ca ²⁺ channel interplay in freshly isolated atrial and ventricular myocytes from the heart of the Japanese quail ( Coturnix japonica ). A trans-sarcolemmal Ca ²⁺ current ( I _Ca ) was detectable in both quail atrial and ventricular myocytes, and was mediated only by L-type Ca ²⁺ channels. The peak density of I _Ca was larger in ventricular cells than in atrial cells, and exceeded that reported for mammalian myocardium recorded under similar conditions. Steady-state SR Ca ²⁺ content of quail myocardium was also larger than that reported for mammals, and reached 750.6±128.2 μmol l ^-1 in atrial cells and 423.3±47.2 μmol l ^-1 in ventricular cells at 24°C. We observed SR Ca ²⁺ -dependent inactivation of I _Ca in ventricular myocytes, indicating cross-talk between sarcolemmal Ca ²⁺ channels and ryanodine receptors in the SR. However, this phenomenon was not observed in atrial myocytes. Taken together, these findings help to explain the high-efficiency avian myocyte excitation-contraction coupling with regard to their reptilian-like cellular ultrastructure.
Competing Interests: Competing interestsThe authors declare no competing or financial interests.
(© 2020. Published by The Company of Biologists Ltd.)