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Vof-16 knockout improves the recovery from hypoxic-ischemic brain damage of neonatal rats.
- Source :
-
Brain research [Brain Res] 2020 Dec 01; Vol. 1748, pp. 147070. Date of Electronic Publication: 2020 Aug 19. - Publication Year :
- 2020
-
Abstract
- Hypoxic-ischemic encephalopathy (HIE) results in high neonatal mortality and severe neurological impairments, and its underlying molecular mechanism underwent extensive investigations. Long non-coding RNA (lncRNA) is considered to be an important regulator on brain development and many neurological diseases. Currently, little is known about the role of Vof-16 (lncRNA) in HIE. We detected the relative expression level of Vof-16 in the cortex and hippocampus of hypoxic-ischemic (HI) models whose successful establishment was verified by TTC staining. Then, Vof-16 knockout rats were generated using the CRISPR/Cas engineering technology to search the specific function of the Vof-16 through a series of behavioral evaluations including Neurological severity scores (NSS), Y-maze test, Morris water maze (MWW) test, open field test, and Rotarod test. The results demonstrated the expression of Vof-16 was substantially up-regulated in the cortex and hippocampus of rats with HI injury. Importantly, Vof-16 knockout facilitated the recovery from long-term HI induced nerve damage and neurobehavioral dysfunctions. In conclusion, this study suggests Vof-16 knockout is a promising treatment target for neonatal HIE.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Animals, Newborn
CRISPR-Cas Systems
Gene Knockout Techniques
Hypoxia-Ischemia, Brain metabolism
Maze Learning physiology
Motor Activity physiology
Proteins metabolism
Rats
Rats, Sprague-Dawley
Brain metabolism
Hypoxia-Ischemia, Brain genetics
Proteins genetics
Recovery of Function physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-6240
- Volume :
- 1748
- Database :
- MEDLINE
- Journal :
- Brain research
- Publication Type :
- Academic Journal
- Accession number :
- 32827549
- Full Text :
- https://doi.org/10.1016/j.brainres.2020.147070