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Time-gated luminescence imaging of positively charged poly-l-lysine-coated highly microporous silicon nanoparticles in living Hydra polyp.
Time-gated luminescence imaging of positively charged poly-l-lysine-coated highly microporous silicon nanoparticles in living Hydra polyp.
- Source :
-
Journal of biophotonics [J Biophotonics] 2020 Dec; Vol. 13 (12), pp. e202000272. Date of Electronic Publication: 2020 Sep 16. - Publication Year :
- 2020
-
Abstract
- The development of non-toxic fluorescent agents alternative to heavy metal-based semiconductor quantum dots represents a relevant topic in biomedical research and in particular in the bioimaging field. Herein, highly luminescent Si─H terminal microporous silicon nanoparticles with μs-lived photoemission are chemically modified with a two step process and successfully used as label-free probes for in vivo time-gated luminescence imaging. In this context, Hydra vulgaris is used as model organism for in vivo study and validity assessment. The application of time gating allows to pursue an effective sorting of the signals, getting rid of the most common sources of noise that are fast-decay tissue autofluorescence and excitation scattering within the tissue. Indeed, an enhancement by a factor ~ 20 in the image signal-to-noise ratio can be estimated.<br /> (© 2020 Wiley-VCH GmbH.)
- Subjects :
- Animals
Luminescence
Polylysine
Silicon
Hydra
Nanoparticles
Subjects
Details
- Language :
- English
- ISSN :
- 1864-0648
- Volume :
- 13
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Journal of biophotonics
- Publication Type :
- Academic Journal
- Accession number :
- 32827195
- Full Text :
- https://doi.org/10.1002/jbio.202000272