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Decidual NK Cells Transfer Granulysin to Selectively Kill Bacteria in Trophoblasts.
- Source :
-
Cell [Cell] 2020 Sep 03; Vol. 182 (5), pp. 1125-1139.e18. Date of Electronic Publication: 2020 Aug 20. - Publication Year :
- 2020
-
Abstract
- Maternal decidual NK (dNK) cells promote placentation, but how they protect against placental infection while maintaining fetal tolerance is unclear. Here we show that human dNK cells highly express the antimicrobial peptide granulysin (GNLY) and selectively transfer it via nanotubes to extravillous trophoblasts to kill intracellular Listeria monocytogenes (Lm) without killing the trophoblast. Transfer of GNLY, but not other cell death-inducing cytotoxic granule proteins, strongly inhibits Lm in human placental cultures and in mouse and human trophoblast cell lines. Placental and fetal Lm loads are lower and pregnancy success is greatly improved in pregnant Lm-infected GNLY-transgenic mice than in wild-type mice that lack GNLY. This immune defense is not restricted to pregnancy; peripheral NK (pNK) cells also transfer GNLY to kill bacteria in macrophages and dendritic cells without killing the host cell. Nanotube transfer of GNLY allows dNK to protect against infection while leaving the maternal-fetal barrier intact.<br />Competing Interests: Declaration of Interests The authors declare no competing interests.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Cell Line
Cell Line, Tumor
Dendritic Cells immunology
Female
HeLa Cells
Humans
Macrophages immunology
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Transgenic
Placenta immunology
Placenta microbiology
Pregnancy
Rats
THP-1 Cells
Trophoblasts microbiology
Antigens, Differentiation, T-Lymphocyte immunology
Bacteria immunology
Cell Movement immunology
Killer Cells, Natural immunology
Trophoblasts immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4172
- Volume :
- 182
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Cell
- Publication Type :
- Academic Journal
- Accession number :
- 32822574
- Full Text :
- https://doi.org/10.1016/j.cell.2020.07.019