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Salinimicrobium nanhaiense sp. nov. and Salinimicrobium oceani sp. nov., two novel species of the family Flavobacteriaceae isolated from the South China Sea.
Salinimicrobium nanhaiense sp. nov. and Salinimicrobium oceani sp. nov., two novel species of the family Flavobacteriaceae isolated from the South China Sea.
- Source :
-
International journal of systematic and evolutionary microbiology [Int J Syst Evol Microbiol] 2020 Oct; Vol. 70 (10), pp. 5263-5270. Date of Electronic Publication: 2020 Aug 21. - Publication Year :
- 2020
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Abstract
- Strains J15B81-2 <superscript>T</superscript> and J15B91 <superscript>T</superscript> were isolated from a sediment sample collected from the South China Sea. Cells of both strains were observed to be rod-shaped, non-gliding, Gram-stain-negative, yellow-pigmented, facultatively anaerobic, catalase-positive, oxidase-negative and showing optimum growth at 30 °C. Strains J15B81-2 <superscript>T</superscript> and J15B91 <superscript>T</superscript> could tolerate up to 9 and 10 % (w/v) NaCl concentration and grow at pH 6.5-9.5 and 6.0-9.0, respectively. The strains shared 97.4 % 16S rRNA gene sequence similarity to each other but were identified as two distinct species based on 81.1-85.8 % ANIb and 31.5 % dDDH values calculated using whole genome sequences. Strains J15B81-2 <superscript>T</superscript> and J15B91 <superscript>T</superscript> shared highest 16S rRNA gene sequence similarity to Salinimicrobium xinjiangense CGMCC 1.12522 <superscript>T</superscript> (98.4 %) and Salinimicrobium sediminis CGMCC 1.12641 <superscript>T</superscript> (98.0 %), respectively. Among species with validly published names, S. sediminis CGMCC 1.12641 <superscript>T</superscript> shared close genetic relatedness with strains J15B81-2 <superscript>T</superscript> [85.1-85.3% average nucleotide identity based on blastBlast+ (ANIb) and 30.6 % digital DNA-DNA hybridization (dDDH)] and J15B91 <superscript>T</superscript> (76.6-79.1 % ANIb and 21.5 % dDDH). The major fatty acid of strains J15B81-2 <superscript>T</superscript> and J15B91 <superscript>T</superscript> were identified as iso-C <subscript>15 : 0</subscript> and iso-C <subscript>16 : 0</subscript> , respectively, and the major polar lipids of the two strains consisted of phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and one unidentified lipid. The strains contained MK-6 as their predominant menaquinone. The genomic G+C contents of strains J15B81-2 <superscript>T</superscript> and J15B91 <superscript>T</superscript> were determined to be 41.7 and 41.8 mol %, respectively. Both strains are considered to represent two novel species of the genus Salinimicrobium and the names Salinimicrobium nanhaiense sp. nov. and Salinimicrobium oceani sp. nov. are proposed for strains J15B81-2 <superscript>T</superscript> (=KCTC 72867 <superscript>T</superscript> =MCCC 1H00410 <superscript>T</superscript> ) and J15B91 <superscript>T</superscript> (=KCTC 72869 <superscript>T</superscript> =MCCC 1H00411 <superscript>T</superscript> ), respectively.
- Subjects :
- Bacterial Typing Techniques
Base Composition
China
DNA, Bacterial genetics
Fatty Acids chemistry
Flavobacteriaceae isolation & purification
Nucleic Acid Hybridization
Phospholipids chemistry
Pigmentation
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Vitamin K 2 analogs & derivatives
Vitamin K 2 chemistry
Flavobacteriaceae classification
Geologic Sediments microbiology
Phylogeny
Seawater microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1466-5034
- Volume :
- 70
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- International journal of systematic and evolutionary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 32821036
- Full Text :
- https://doi.org/10.1099/ijsem.0.004405