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Loss of the seipin gene perturbs eggshell formation in Caenorhabditis elegans .
- Source :
-
Development (Cambridge, England) [Development] 2020 Oct 16; Vol. 147 (20). Date of Electronic Publication: 2020 Oct 16. - Publication Year :
- 2020
-
Abstract
- Seipin, an evolutionary conserved protein, plays pivotal roles during lipid droplet (LD) biogenesis and is associated with various human diseases with unclear mechanisms. Here, we analyzed Caenorhabditis elegans mutants deleted of the sole SEIPIN gene, seip-1 Homozygous seip-1 mutants displayed penetrant embryonic lethality, which is caused by the disruption of the lipid-rich permeability barrier, the innermost layer of the C. elegans embryonic eggshell. In C. elegans oocytes and embryos, SEIP-1 is associated with LDs and is crucial for controlling LD size and lipid homeostasis. The seip-1 deletion mutants reduced the ratio of polyunsaturated fatty acids (PUFAs) in their embryonic fatty acid pool. Interestingly, dietary supplementation of selected n-6 PUFAs rescued the embryonic lethality and defective permeability barrier. Accordingly, we propose that SEIP-1 may maternally regulate LD biogenesis and lipid homeostasis to orchestrate the formation of the permeability barrier for eggshell synthesis during embryogenesis. A lipodystrophy allele of seip-1 resulted in embryonic lethality as well and could be rescued by PUFA supplementation. These experiments support a great potential for using C. elegans to model SEIPIN-associated human diseases.<br />Competing Interests: Competing interestsThe authors declare no competing or financial interests.<br /> (© 2020. Published by The Company of Biologists Ltd.)
- Subjects :
- Animals
Caenorhabditis elegans drug effects
Caenorhabditis elegans ultrastructure
Caenorhabditis elegans Proteins metabolism
Dietary Supplements
Disease Models, Animal
Egg Shell drug effects
Egg Shell ultrastructure
Embryo, Nonmammalian drug effects
Embryo, Nonmammalian metabolism
Embryo, Nonmammalian ultrastructure
Fatty Acids, Unsaturated pharmacology
Fertilization
Gene Deletion
Gene Expression Regulation, Developmental drug effects
Humans
Lipid Droplets metabolism
Lipid Droplets ultrastructure
Lipidomics
Membrane Proteins metabolism
Mutation genetics
Oocytes drug effects
Oocytes metabolism
Oocytes ultrastructure
Ovulation drug effects
Permeability
Saccharomyces cerevisiae genetics
Caenorhabditis elegans embryology
Caenorhabditis elegans genetics
Caenorhabditis elegans Proteins genetics
Egg Shell embryology
Genes, Helminth
Membrane Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9129
- Volume :
- 147
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- Development (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 32820022
- Full Text :
- https://doi.org/10.1242/dev.192997