Ribosome-Mediated Attenuation of vga (A) Expression Is Shaped by the Antibiotic Resistance Specificity of Vga(A) Protein Variants.

Authors :: Vimberg V
Cavanagh JP
Novotna M
Lenart J
Nguyen Thi Ngoc B
Vesela J
Pain M
Koberska M
Balikova Novotna G
Source :: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2020 Oct 20; Vol. 64 (11). Date of Electronic Publication: 2020 Oct 20 (Print Publication: 2020).
Publication Year :: 2020
Abstract: Vga(A) protein variants confer different levels of resistance to lincosamides, streptogramin A, and pleuromutilins (LS <subscript>A</subscript> P) by displacing antibiotics from the ribosome. Here, we show that expression of vga (A) variants from Staphylococcus haemolyticus is regulated by cis -regulatory RNA in response to the LS <subscript>A</subscript> P antibiotics by the mechanism of ribosome-mediated attenuation. The specificity of induction depends on Vga(A)-mediated resistance rather than on the sequence of the riboregulator. Fine tuning between Vga(A) activity and its expression in response to the antibiotics may contribute to the selection of more potent Vga(A) variants because newly acquired mutation can be immediately phenotypically manifested.<br /> (Copyright © 2020 American Society for Microbiology.)

Subjects :: Anti-Bacterial Agents pharmacology
Bacterial Proteins genetics
Lincosamides
Macrolides
Ribosomes genetics
Drug Resistance, Multiple, Bacterial
Streptogramin A

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