Rhodovarius crocodyli sp. nov., isolated from a crocodile pond.

Bacterial strain CCP-6 ^T , isolated from a freshwater pond in Taiwan, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain CCP-6 ^T is affiliated with species in the genus Rhodovarius . Strain CCP-6 ^T was most closely related to Rhodovarius lipocyclicus CCUG 44693 ^T with a 98.9% 16S rRNA gene sequence similarity. Cells were Gram-stain-negative, aerobic, non-motile, rod-shaped and formed light pink-coloured colonies. Optimal growth occurred at 30 °C, pH 6 and in the absence of NaCl. The major fatty acids of strain CCP-6 ^T were C _{18 : 1} ω 7 c , C _{16 : 0} and C _{19 : 0} cyclo ω 8 c . The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidyldimethylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, three unidentified aminophospholipids and an unidentified phospholipid. The predominant polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content of the genomic DNA was 69.3 mol%. Strain CCP-6 ^T showed 85.8% average nucleotide identity and 14.5% digital DNA-DNA hybridization identity with Rhodovarius lipocyclicus CCUG 44693 ^T . On the basis of the genotypic, chemotaxonomic and phenotypic data, strain CCP-6 ^T represents a novel species in the genus Rhodovarius , for which the name Rhodovarius crocodyli sp. nov. is proposed. The type strain is CCP-6 ^T (=BCRC 81095 ^T =LMG 30310 ^T =KCTC 62188 ^T ).