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MICAL2 is essential for myogenic lineage commitment.
- Source :
-
Cell death & disease [Cell Death Dis] 2020 Aug 18; Vol. 11 (8), pp. 654. Date of Electronic Publication: 2020 Aug 18. - Publication Year :
- 2020
-
Abstract
- Contractile myofiber units are mainly composed of thick myosin and thin actin (F-actin) filaments. F-Actin interacts with Microtubule Associated Monooxygenase, Calponin And LIM Domain Containing 2 (MICAL2). Indeed, MICAL2 modifies actin subunits and promotes actin filament turnover by severing them and preventing repolymerization. In this study, we found that MICAL2 increases during myogenic differentiation of adult and pluripotent stem cells (PSCs) towards skeletal, smooth and cardiac muscle cells and localizes in the nucleus of acute and chronic regenerating muscle fibers. In vivo delivery of Cas9-Mical2 guide RNA complexes results in muscle actin defects and demonstrates that MICAL2 is essential for skeletal muscle homeostasis and functionality. Conversely, MICAL2 upregulation shows a positive impact on skeletal and cardiac muscle commitments. Taken together these data demonstrate that modulations of MICAL2 have an impact on muscle filament dynamics and its fine-tuned balance is essential for the regeneration of muscle tissues.
- Subjects :
- Actin Cytoskeleton metabolism
Actin Cytoskeleton physiology
Actins metabolism
Actins physiology
Animals
Cell Differentiation physiology
Cytoskeletal Proteins physiology
Cytoskeleton metabolism
Female
Male
Mice
Mice, Inbred C57BL
Muscle Development physiology
Muscle, Skeletal metabolism
Muscle, Smooth physiology
Myosins physiology
Cytoskeletal Proteins metabolism
Muscle Contraction physiology
Myosins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-4889
- Volume :
- 11
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Cell death & disease
- Publication Type :
- Academic Journal
- Accession number :
- 32811811
- Full Text :
- https://doi.org/10.1038/s41419-020-02886-z