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Dual-Color Single-Cell Imaging of the Suprachiasmatic Nucleus Reveals a Circadian Role in Network Synchrony.

Authors :
Shan Y
Abel JH
Li Y
Izumo M
Cox KH
Jeong B
Yoo SH
Olson DP
Doyle FJ 3rd
Takahashi JS
Source :
Neuron [Neuron] 2020 Oct 14; Vol. 108 (1), pp. 164-179.e7. Date of Electronic Publication: 2020 Aug 07.
Publication Year :
2020

Abstract

The suprachiasmatic nucleus (SCN) acts as a master pacemaker driving circadian behavior and physiology. Although the SCN is small, it is composed of many cell types, making it difficult to study the roles of particular cells. Here we develop bioluminescent circadian reporter mice that are Cre dependent, allowing the circadian properties of genetically defined populations of cells to be studied in real time. Using a Color-Switch PER2::LUCIFERASE reporter that switches from red PER2::LUCIFERASE to green PER2::LUCIFERASE upon Cre recombination, we assess circadian rhythms in two of the major classes of peptidergic neurons in the SCN: AVP (arginine vasopressin) and VIP (vasoactive intestinal polypeptide). Surprisingly, we find that circadian function in AVP neurons, not VIP neurons, is essential for autonomous network synchrony of the SCN and stability of circadian rhythmicity.<br />Competing Interests: Declaration of Interests The authors declare no competing interests.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1097-4199
Volume :
108
Issue :
1
Database :
MEDLINE
Journal :
Neuron
Publication Type :
Academic Journal
Accession number :
32768389
Full Text :
https://doi.org/10.1016/j.neuron.2020.07.012