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Dual-Color Single-Cell Imaging of the Suprachiasmatic Nucleus Reveals a Circadian Role in Network Synchrony.
- Source :
-
Neuron [Neuron] 2020 Oct 14; Vol. 108 (1), pp. 164-179.e7. Date of Electronic Publication: 2020 Aug 07. - Publication Year :
- 2020
-
Abstract
- The suprachiasmatic nucleus (SCN) acts as a master pacemaker driving circadian behavior and physiology. Although the SCN is small, it is composed of many cell types, making it difficult to study the roles of particular cells. Here we develop bioluminescent circadian reporter mice that are Cre dependent, allowing the circadian properties of genetically defined populations of cells to be studied in real time. Using a Color-Switch PER2::LUCIFERASE reporter that switches from red PER2::LUCIFERASE to green PER2::LUCIFERASE upon Cre recombination, we assess circadian rhythms in two of the major classes of peptidergic neurons in the SCN: AVP (arginine vasopressin) and VIP (vasoactive intestinal polypeptide). Surprisingly, we find that circadian function in AVP neurons, not VIP neurons, is essential for autonomous network synchrony of the SCN and stability of circadian rhythmicity.<br />Competing Interests: Declaration of Interests The authors declare no competing interests.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- ARNTL Transcription Factors genetics
Animals
Gene Knockout Techniques
Luciferases
Mice
Mice, Transgenic
Period Circadian Proteins metabolism
Single-Cell Analysis
Suprachiasmatic Nucleus metabolism
Time-Lapse Imaging
Arginine Vasopressin metabolism
Circadian Rhythm
Nerve Net metabolism
Period Circadian Proteins genetics
Suprachiasmatic Nucleus Neurons metabolism
Vasoactive Intestinal Peptide metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4199
- Volume :
- 108
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neuron
- Publication Type :
- Academic Journal
- Accession number :
- 32768389
- Full Text :
- https://doi.org/10.1016/j.neuron.2020.07.012