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Determination of optimal parameters for 3D single-point macromolecular proton fraction mapping at 7T in healthy and demyelinated mouse brain.
- Source :
-
Magnetic resonance in medicine [Magn Reson Med] 2021 Jan; Vol. 85 (1), pp. 369-379. Date of Electronic Publication: 2020 Jul 27. - Publication Year :
- 2021
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Abstract
- Purpose: To determine optimal constrained tissue parameters and off-resonance sequence parameters for single-point macromolecular proton fraction (SP-MPF) mapping based on a comprehensive quantitative magnetization transfer (qMT) protocol in healthy and demyelinated living mice at 7T.<br />Methods: Using 3D spoiled gradient echo-based sequences, a comprehensive qMT protocol is performed by sampling the Z-spectrum of mice brains, in vivo. Provided additional T <subscript>1</subscript> , B 1 + and B <subscript>0</subscript> maps allow for the estimation of qMT tissue parameters, among which three will be constrained, namely the longitudinal and transverse relaxation characteristics of the free pool (R <subscript>1,f</subscript> T <subscript>2,f</subscript> ), the cross-relaxation rate (R) and the bound pool transverse relaxation time (T <subscript>2,r</subscript> ). Different sets of constrained parameters are investigated to reduce the bias between the SP-MPF and its reference based on the comprehensive protocol.<br />Results: Based on a whole-brain histogram analysis about the constrained parameters, the optimal experimental parameters that minimize the global bias between reference and SP-MPF maps consist of a 600° and 6 kHz off-resonance irradiation pulse. Following a Bland-Altman analysis over regions of interest, optimal constrained parameters were R <subscript>1,f</subscript> T <subscript>2,f </subscript> = 0.0129, R = 26.5 s <superscript>-1</superscript> , and T <subscript>2,r </subscript> = 9.1 µs, yielding an overall MPF bias of 10 <superscript>-4</superscript> (limits of agreement [-0.0068;0.0070]) and a relative variation of 0.64% ± 5.95% between the reference and the optimal single-point method across all mice.<br />Conclusion: The necessity of estimating animal model- and field-dependent constrained parameters was demonstrated. The single-point MPF method can be reliably applied at 7T, as part of routine preclinical in vivo imaging protocol in mice.<br /> (© 2020 International Society for Magnetic Resonance in Medicine.)
Details
- Language :
- English
- ISSN :
- 1522-2594
- Volume :
- 85
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Magnetic resonance in medicine
- Publication Type :
- Academic Journal
- Accession number :
- 32767495
- Full Text :
- https://doi.org/10.1002/mrm.28397