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Auxin-induced signaling protein nanoclustering contributes to cell polarity formation.
- Source :
-
Nature communications [Nat Commun] 2020 Aug 06; Vol. 11 (1), pp. 3914. Date of Electronic Publication: 2020 Aug 06. - Publication Year :
- 2020
-
Abstract
- Cell polarity is fundamental to the development of both eukaryotes and prokaryotes, yet the mechanisms behind its formation are not well understood. Here we found that, phytohormone auxin-induced, sterol-dependent nanoclustering of cell surface transmembrane receptor kinase 1 (TMK1) is critical for the formation of polarized domains at the plasma membrane (PM) during the morphogenesis of cotyledon pavement cells (PC) in Arabidopsis. Auxin-induced TMK1 nanoclustering stabilizes flotillin1-associated ordered nanodomains, which in turn promote the nanoclustering of ROP6 GTPase that acts downstream of TMK1 to regulate cortical microtubule organization. In turn, cortical microtubules further stabilize TMK1- and flotillin1-containing nanoclusters at the PM. Hence, we propose a new paradigm for polarity formation: A diffusive signal triggers cell polarization by promoting cell surface receptor-mediated nanoclustering of signaling components and cytoskeleton-mediated positive feedback that reinforces these nanodomains into polarized domains.
- Subjects :
- Arabidopsis genetics
Arabidopsis Proteins chemistry
Cell Membrane metabolism
Cell Polarity genetics
Lipid Metabolism
Membrane Proteins chemistry
Membrane Proteins metabolism
Models, Biological
Monomeric GTP-Binding Proteins chemistry
Monomeric GTP-Binding Proteins metabolism
Mutation
Plant Growth Regulators metabolism
Plants, Genetically Modified
Protein Aggregates
Protein Serine-Threonine Kinases chemistry
Protein Stability
Signal Transduction
Arabidopsis cytology
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Cell Polarity physiology
Indoleacetic Acids metabolism
Protein Serine-Threonine Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 32764676
- Full Text :
- https://doi.org/10.1038/s41467-020-17602-w