Back to Search
Start Over
Screening of potential uranium protein targets in fish ovaries after chronic waterborne exposure: Differences and similarities between roach and zebrafish.
- Source :
-
Journal of environmental radioactivity [J Environ Radioact] 2020 Oct; Vol. 222, pp. 106365. Date of Electronic Publication: 2020 Aug 01. - Publication Year :
- 2020
-
Abstract
- Concentration of uranium (U), a naturally encountered radioactive element in earth's crust, can be enhanced in freshwater ecosystems (μg.L <superscript>-1</superscript> - mg.L <superscript>-1</superscript> ) due to various anthropogenic activities. The consequent aquatic organism exposure to U leads to its accumulation in all organs, particularly in the gonad, and in subcellular fractions (mainly the cytosol); then it is known to affect fish at several biological levels, and more particularly, at a reproduction endpoint, with a decrease in the total number of eggs, spawn events and larvae survival. The understanding of U reprotoxicity requires the fine knowledge of its speciation at molecular level, i.e., its interaction with cytosolic biomolecules. In this study, we focus on the U-protein interactions in gonads. A non-denaturating extraction protocol combined with size exclusion chromatography (SEC) allowed the separation of metal-protein complexes in ovaries of U-contaminated wild roaches before their elemental detection (ICP MS). This enables unprecedented information to be obtained about U distribution in ovaries of autochthonous fish, Rutilus rutilus, which is different in some points from that obtained in the model species, Danio rerio under controlled laboratory conditions at a similar concentration level. Finally, the ability to transpose results from model to autochthonous fish was briefly discussed.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1879-1700
- Volume :
- 222
- Database :
- MEDLINE
- Journal :
- Journal of environmental radioactivity
- Publication Type :
- Academic Journal
- Accession number :
- 32750598
- Full Text :
- https://doi.org/10.1016/j.jenvrad.2020.106365