Hymenobacter fodinae sp. nov. and Hymenobacter metallicola sp. nov., isolated from abandoned lead-zinc mine.

Two novel strains, designated 92R-1 ^T and 9PBR-1 ^T , were isolated from abandoned lead-zinc ore collected in Meizhou, Guangdong Province, PR China. Phylogenetic analyses based on 16S rRNA gene sequences showed that they fell into the genus of Hymenobacte r and formed two distinct lineages. Strain 92R-1 ^T was most closely related to Hymenobacter wooponensis JCM 19491 ^T (98.7 %) and Hymenobacter gelipurpurascens LMG 21873 ^T (98.5 %), while strain 9PBR-1 ^T was most closely related to Hymenobacter chitinivorans LMG 21951 ^T (99.0 %), Hymenobacter elongatus JCM 17223 ^T (98.7 %) and Hymenobacter aquaticus JCM 31653 ^T (98.1 %). Strain 92R-1 ^T shared average nucleotide identity values of 80.0-83.7 % and digital DNA-DNA hybridization values of 23.1-27.1 % with its closely related type strains, respectively, while strain 9PBR-1 ^T shared corresponding values of 80.3-83.2 % and 23.6-26.7 % with its closely related type strains, respectively. The two novel strains could be clearly distinguished from their closely related type strains by enzyme activities and substrates assimilation, respectively. Both of them took iso-C _15:0, summed feature 3 (C _16:1 ω 7 c and/or C _16:1 ω 6 c ), summed feature 4 (iso-C _17:1 I and/or anteiso-C _17:1 B) and C _16:1 ω 5 c as major fatty acids, and showed clear differences from their closely relatives in the contents of several components. They contained menaquinone 7 as the major respiratory quinone and phosphatidylethanolamine as the dominant polar lipid. The G+C contents of strains 92R-1 ^T and 9PBR-1 ^T were 56.7 and 59.5 mol%, respectively. The results clearly supported that strains 92R-1 ^T and 9PBR-1 ^T represent two distinct novel species within the genus Hymenobacter , for which the names Hymenobacter fodinae sp. nov. (type strain 92R-1 ^T =GDMCC 1.1493 ^T =JCM 32697 ^T ) and Hymenobacter metallicola sp. nov. (type strain 9PBR-1 ^T =GDMCC 1.1491 ^T =JCM 32698 ^T ) are proposed.