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Conjugative plasmidic AmpC detected in Escherichia coli, Proteus mirabilis and Klebsiella pneumoniae human clinical isolates from Portugal.
- Source :
-
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2020 Dec; Vol. 51 (4), pp. 1807-1812. Date of Electronic Publication: 2020 Aug 01. - Publication Year :
- 2020
-
Abstract
- AmpC is a type of β-lactamase enzyme produced by bacteria; these enzymes are classified in Class C and Group 1, and these confer resistance to cephamycin. Enterobacterales producing AmpC are reported worldwide and have great clinical importance due to therapeutic restriction and epidemiological importance once the easy dissemination by plasmidic genes to other bacteria is a real threat. These genes are naturally found in some enterobacteria as Enterobacter cloacae, Morganella morganii, and Citrobacter freundii, but other species have demonstrated similar resistance phenotype of AmpC production. Genes carried in plasmids have been described in these species conferring resistance to cefoxitin and causing therapeutic failure in some bacterial infections. This work detected and described five clinical strains of Escherichia coli, Proteus mirabilis, and Klebsiella pneumoniae that presented plasmid ampC (pAmpC) isolated from the north of Portugal collected in 2009. AmpC production was confirmed by inhibition of the enzyme by cloxacillin and boronic acid in agar diffusion tests. Also, PCR (polymerase chain reaction) was performed for the detection of gene universal to AmpC, bla <subscript>ampC</subscript> , and others to AmpC group: bla <subscript>ACC</subscript> , bla <subscript>CIT</subscript> , bla <subscript>CMY</subscript> , bla <subscript>DHA</subscript> , and bla <subscript>EBC</subscript> . The conjugation in liquid medium for 24 h was realized to determine if gene is localized in chromosome or plasmid. The isolates and their conjugants showed phenotypic characteristics and bla <subscript>CMY</subscript> and bla <subscript>CIT</subscript> were detected by PCR corroborating the AmpC characteristics observed in these bacteria. Confirmation of transfer of plasmid containing genes encoding AmpC is of high epidemiological relevance to the hospital studied and demonstrated the importance of AmpC surveillance and studies in hospital and community environments in order to choose the appropriate therapy for bacterial infections.
- Subjects :
- Anti-Bacterial Agents pharmacology
Bacterial Proteins metabolism
Boronic Acids pharmacology
Cloxacillin pharmacology
Drug Resistance, Multiple, Bacterial drug effects
Drug Resistance, Multiple, Bacterial genetics
Enterobacteriaceae classification
Enterobacteriaceae drug effects
Enterobacteriaceae isolation & purification
Enterobacteriaceae Infections epidemiology
Enterobacteriaceae Infections microbiology
Humans
Microbial Sensitivity Tests
Portugal epidemiology
beta-Lactamases metabolism
Bacterial Proteins genetics
Conjugation, Genetic
Enterobacteriaceae genetics
Plasmids genetics
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1678-4405
- Volume :
- 51
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
- Publication Type :
- Academic Journal
- Accession number :
- 32740783
- Full Text :
- https://doi.org/10.1007/s42770-020-00355-5