Back to Search Start Over

Human Respiratory Syncytial Virus-Induced Immune Signature of Infection Revealed by Transcriptome Analysis of Clinical Pediatric Nasopharyngeal Swab Samples.

Authors :
Nicolas De Lamballerie C
Pizzorno A
Dubois J
Padey B
Julien T
Traversier A
Carbonneau J
Orcel E
Lina B
Hamelin ME
Roche M
Textoris J
Boivin G
Legras-Lachuer C
Terrier O
Rosa-Calatrava M
Source :
The Journal of infectious diseases [J Infect Dis] 2021 Mar 29; Vol. 223 (6), pp. 1052-1061.
Publication Year :
2021

Abstract

Human respiratory syncytial virus (HRSV) constitutes one the main causes of respiratory infection in neonates and infants worldwide. Transcriptome analysis of clinical samples using high-throughput technologies remains an important tool to better understand virus-host complex interactions in the real-life setting but also to identify new diagnosis/prognosis markers or therapeutics targets. A major challenge when exploiting clinical samples such as nasal swabs, washes, or bronchoalveolar lavages is the poor quantity and integrity of nucleic acids. In this study, we applied a tailored transcriptomics workflow to exploit nasal wash samples from children who tested positive for HRSV. Our analysis revealed a characteristic immune signature as a direct reflection of HRSV pathogenesis and highlighted putative biomarkers of interest such as IP-10, TMEM190, MCEMP1, and TIMM23.<br /> (© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.)

Details

Language :
English
ISSN :
1537-6613
Volume :
223
Issue :
6
Database :
MEDLINE
Journal :
The Journal of infectious diseases
Publication Type :
Academic Journal
Accession number :
32726438
Full Text :
https://doi.org/10.1093/infdis/jiaa468