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Efficient construction of a stable linear gene based on a TNA loop modified primer pair for gene delivery.
- Source :
-
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2020 Aug 25; Vol. 56 (68), pp. 9894-9897. - Publication Year :
- 2020
-
Abstract
- A terminal-closed linear gene with strong exonuclease resistance and serum stability was successfully constructed by polymerase chain reaction (PCR) with an α-l-threose nucleic acid (TNA) loop modified primer pair, which can be used as an efficient gene expression system in eukaryotic cells for gene delivery.
- Subjects :
- DNA Primers metabolism
Gene Expression
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
HEK293 Cells
Humans
Microscopy, Fluorescence
Plasmids genetics
Plasmids metabolism
Polymerase Chain Reaction
DNA Primers chemistry
Nucleic Acids chemistry
Tetroses chemistry
Transfection methods
Subjects
Details
- Language :
- English
- ISSN :
- 1364-548X
- Volume :
- 56
- Issue :
- 68
- Database :
- MEDLINE
- Journal :
- Chemical communications (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 32720666
- Full Text :
- https://doi.org/10.1039/d0cc04356g