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Seminal protein levels assessed with dipstick test correlate with sperm recovery after cryopreservation.
- Source :
-
Human fertility (Cambridge, England) [Hum Fertil (Camb)] 2020 Jul 25, pp. 1-7. Date of Electronic Publication: 2020 Jul 25. - Publication Year :
- 2020
- Publisher :
- Ahead of Print
-
Abstract
- The process of freezing/thawing causes structural and functional damage to sperm samples, which can be mitigated by seminal plasma proteins. This study investigated the proposition that seminal protein measurements using a urinary dipstick prior to freezing could help predict the post-thaw recovery of live spermatozoa. This was a prospective study including 149 men undergoing semen analysis due to male and/or female infertility. The seminal samples were analysed according to World Health Organisation standards and protein concentrations were measured using a commercially available urinary dipstick following quantitative validation. The median live sperm recovery rates were 79%, 81% and 94%, respectively, in samples with protein concentrations of ≤1.0 g/L (+/++), 3.0 g/L (+++) and ≥20.0 g/L (++++) measured in fresh specimen dipstick analysis ( p < 0.05) indicating that the probability of recovering at least 50% of frozen spermatozoa increased progressively with higher protein concentrations in the fresh sample (chi-square for linear association = 7.17. p = 0.007). In conclusion, fresh seminal protein concentration levels assessed with a dipstick test correlate with the proportion of live spermatozoa recovered from cryopreserved samples. This simple, low-cost test may add prognostic information to baseline semen analysis prior to sperm banking.
Details
- Language :
- English
- ISSN :
- 1742-8149
- Database :
- MEDLINE
- Journal :
- Human fertility (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 32715868
- Full Text :
- https://doi.org/10.1080/14647273.2020.1798515