Flow cytometric identification of T fh 13 cells in mouse and human.

Authors :: Chen JS
Grassmann JDS
Gowthaman U
Olyha SJ
Simoneau T
Berin MC
Eisenbarth SC
Williams A
Source :: The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 2021 Feb; Vol. 147 (2), pp. 470-483. Date of Electronic Publication: 2020 Jul 21.
Publication Year :: 2021
Abstract: Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper T <subscript>fh</subscript> 13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying T <subscript>fh</subscript> 13 cells in both mice and humans.<br /> (Copyright © 2020 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)