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Construction of Ginsenoside Nanoparticles with pH/Reduction Dual Response for Enhancement of Their Cytotoxicity Toward HepG2 Cells.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2020 Aug 12; Vol. 68 (32), pp. 8545-8556. Date of Electronic Publication: 2020 Aug 03. - Publication Year :
- 2020
-
Abstract
- The aim of this study is to construct a pH- and reduction-responsive nanodrug delivery system to effectively deliver a ginsenoside (Rh <subscript>2</subscript> ) and enhance its cytotoxicity against human hepatocarcinoma cells (HepG2). Here, pullulan polysaccharide was grafted by urocanic acid and α-lipoic acid (α-LA) to obtain a copolymer, α-LA-conjugated N -urocanyl pullulan (LA-URPA), which was expected to have pH and redox dual response. Then, the copolymer LA-URPA was used to encapsulate ginsenoside Rh <subscript>2</subscript> to form Rh <subscript>2</subscript> nanoparticles (Rh <subscript>2</subscript> NPs). The results showed that Rh <subscript>2</subscript> NPs exhibited an average size of 119.87 nm with a uniform spherical morphology. Of note, Rh <subscript>2</subscript> NPs showed a high encapsulation efficiency of 86.00%. Moreover, Rh <subscript>2</subscript> NPs possessed excellent pH/reduction dual-responsive drug release under acidic conditions (pH 5.5) and glutathione (GSH) stimulation with a low drug leakage of 14.8% within 96 h. Furthermore, Rh <subscript>2</subscript> NPs with pH/reduction dual response had higher cytotoxicity than Rh <subscript>2</subscript> after incubation with HepG2 cells for 72 h, indicating that Rh <subscript>2</subscript> NPs had a longer circulation time. After the treatment with Rh <subscript>2</subscript> NPs, the excessive increase of reactive oxygen species and the decrease of superoxide dismutase, glutathione (GSH), and mitochondrial membrane potential suggested that the mitochondrial pathway mediated by oxidative stress played a role in this Rh <subscript>2</subscript> NP-induced apoptosis. In conclusion, this study provides a new strategy for improving the application of ginsenoside Rh <subscript>2</subscript> in the food and pharmaceutical fields.
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 68
- Issue :
- 32
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32686932
- Full Text :
- https://doi.org/10.1021/acs.jafc.0c03698