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Comprehensive transcriptome and proteome analyses reveal a novel sodium chloride responsive gene network in maize seed tissues during germination.
- Source :
-
Plant, cell & environment [Plant Cell Environ] 2021 Jan; Vol. 44 (1), pp. 88-101. Date of Electronic Publication: 2020 Oct 28. - Publication Year :
- 2021
-
Abstract
- Germination is a plant developmental process by which radicle of mature seeds start to penetrate surrounding barriers for seedling establishment and multiple environmental factors have been shown to affect it. Little is known how high salinity affects seed germination of C4 plant, Zea mays. Preliminary germination assay suggested that isolated embryo alone was able to germinate under 200 mM NaCl treatment, whereas the intact seeds were highly repressed. We hypothesized that maize endosperm may function in perception and transduction of salt signal to surrounding tissues such as embryo, showing a completely different response to that in Arabidopsis. Since salt response involves ABA, we analysed in vivo ABA distribution and quantity and the result demonstrated that ABA level in isolated embryo under NaCl treatment failed to increase in comparison with the water control, suggesting that the elevation of ABA level is an endosperm dependent process. Subsequently, by using advanced profiling techniques such as RNA sequencing and SWATH-MS-based quantitative proteomics, we found substantial differences in post-transcriptional and translational changes between salt-treated embryo and endosperm. In summary, our results indicate that these regulatory mechanisms, such as alternative splicing, are likely to mediate early responses to salt stress during maize seed germination.<br /> (© 2020 John Wiley & Sons Ltd.)
- Subjects :
- Abscisic Acid metabolism
Gene Expression Profiling
Gene Regulatory Networks genetics
Gene Regulatory Networks physiology
Gibberellins metabolism
Plant Growth Regulators metabolism
Proteome
Salt Stress
Seeds growth & development
Zea mays growth & development
Zea mays metabolism
Seeds metabolism
Sodium Chloride metabolism
Zea mays genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1365-3040
- Volume :
- 44
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Plant, cell & environment
- Publication Type :
- Academic Journal
- Accession number :
- 32677712
- Full Text :
- https://doi.org/10.1111/pce.13849