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Molecular imaging of cardiac CXCR4 expression in a mouse model of acute myocardial infarction using a novel 68 Ga-mCXCL12 PET tracer.
- Source :
-
Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology [J Nucl Cardiol] 2021 Dec; Vol. 28 (6), pp. 2965-2975. Date of Electronic Publication: 2020 Jul 16. - Publication Year :
- 2021
-
Abstract
- Background: The chemokine receptor CXCR4 and its ligand CXCL12 have been shown to be a possible imaging and therapeutic target after myocardial infarction (MI). The murine-based and mouse-specific <superscript>68</superscript> Ga-mCXCL12 PET tracer could be suitable for serial in vivo quantification of cardiac CXCR4 expression in a murine model of MI.<br />Methods and Results: At days 1-6 after MI, mice were intravenously injected with <superscript>68</superscript> Ga-mCXCL12. Autoradiography was performed and the infarct-to-remote ratio (I/R) was determined. In vivo PET imaging with <superscript>68</superscript> Ga-mCXCL12 was conducted on days 1-6 after MI and the percentage of the injected dose (%ID/g) of the tracer uptake in the infarct area was calculated. <superscript>18</superscript> F-FDG-PET was performed for anatomical landmarking. Ex vivo autoradiography identified CXCR4 upregulation in the infarct region with an increasing I/R after 12 hours (1.4 ± 0.3), showing a significant increase until day 2 (4.5 ± 0.6), followed by a plateau phase (day 4) and decrease after 10 days (1.3 ± 1.0). In vivo PET imaging identified similar CXCR4 upregulation in the infarct region which peaked around day 3 post MI (9.7 ± 5.0 %ID/g) and then subsequently decreased by day 6 (2.8 ± 1.0 %ID/g).<br />Conclusion: Noninvasive molecular imaging of cardiac CXCR4 expression using a novel, murine-based, and specific <superscript>68</superscript> Ga-mCXCL12 tracer is feasible both ex vivo and in vivo.<br /> (© 2020. The Author(s).)
Details
- Language :
- English
- ISSN :
- 1532-6551
- Volume :
- 28
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology
- Publication Type :
- Academic Journal
- Accession number :
- 32676914
- Full Text :
- https://doi.org/10.1007/s12350-020-02262-6