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Effects of two kinds of fishery drugs on the expressions of GAD and GABA-T mRNA in crucian carp (Carassius auratus gibelio).
- Source :
-
Fish physiology and biochemistry [Fish Physiol Biochem] 2020 Dec; Vol. 46 (6), pp. 1965-1973. Date of Electronic Publication: 2020 Jul 15. - Publication Year :
- 2020
-
Abstract
- The objective of this study was to investigate the effects of difloxacin (DIF) and avermectin (AVM) on glutamate decarboxylase (GAD) and GABA-transaminase (GABA-T) in different tissues of crucian carp (Carassius auratus gibelio). After the treatments of DIF and AVM, the mRNA expressions of GAD and GABA-T in different tissues were detected by quantitative real-time PCR (qPCR). The results showed that the mRNA expressions of GAD <subscript>65</subscript> , GAD <subscript>67</subscript> , and GABA-T in the telencephalon (Tel), mesencephalon (Mes), cerebella (Cer), and medulla oblongata (Med) were downregulated significantly with the safe dose (SD, 20 mg/kg) of DIF (P < 0.05 or P < 0.01). While the expressions of GAD <subscript>65</subscript> and GAD <subscript>67</subscript> in the kidney at 12 h had strikingly upregulated to 13.81 ± 1.06** and 150.67 ± 12.85** times. Treated with the lethal dose of 50% (LD <subscript>50</subscript> , 2840 mg/kg b. W.) of DIF, the mRNA expressions of GAD <subscript>65</subscript> , GAD <subscript>67</subscript> , and GABA-T in all tissues were increased significantly (P < 0.01). The results of AVM group showed that the mRNA expressions of GAD <subscript>65</subscript> , GAD <subscript>67</subscript> , and GABA-T both in the central and peripheral tissues were all remarkably downregulated at the safe concentration (SC, 0.0039 mg/L) and the lethal concentration of 50% (LC <subscript>50</subscript> , 0.039 mg/L), except for the mRNA inhibitions of GAD <subscript>65</subscript> , GAD <subscript>67</subscript> , and GABA-T in the muscle at 2 h which sharply downregulated to 0.20 ± 0.02 <superscript>ΔΔ</superscript> × 10 <superscript>-2</superscript> , 0.57 ± 0.06 <superscript>ΔΔ</superscript> × 10 <superscript>-1</superscript> and 0.44 ± 0.02 <superscript>ΔΔ</superscript> × 10 <superscript>-1</superscript> , respectively (P < 0.01).
- Subjects :
- Animals
Brain drug effects
Brain metabolism
Ciprofloxacin pharmacology
Fisheries
Ivermectin pharmacology
Kidney drug effects
Kidney metabolism
Liver drug effects
Liver metabolism
Muscles drug effects
Muscles metabolism
RNA, Messenger metabolism
4-Aminobutyrate Transaminase genetics
Anti-Bacterial Agents pharmacology
Antiprotozoal Agents pharmacology
Carps genetics
Ciprofloxacin analogs & derivatives
Glutamate Decarboxylase genetics
Ivermectin analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1573-5168
- Volume :
- 46
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Fish physiology and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32671610
- Full Text :
- https://doi.org/10.1007/s10695-020-00847-z