Back to Search Start Over

Novel spiking methods developed for anion exchange chromatography operating in a continuous process.

Authors :
Li Y
Chang A
Beattie D
Remington KM
Source :
Biotechnology and bioengineering [Biotechnol Bioeng] 2020 Nov; Vol. 117 (11), pp. 3379-3389. Date of Electronic Publication: 2020 Jul 30.
Publication Year :
2020

Abstract

Many manufacturers of biopharmaceuticals are moving from batch to continuous processing. While this approach offers advantages over batch processing, demonstration of viral clearance for continuous processes is challenging. Fluctuating output from a continuous process chromatography column results in a nonhomogeneous load for the subsequent column and must be considered when designing viral clearance studies. One approach to clearance studies is to downscale the connected unit operations and introduce virus by in-line spiking. This is challenging to be implemented at the contract research organization performing the clearance study given the complexity of systems and level of expertise required. Alternately, each unit operation could be evaluated in traditional batch mode but the spiking and loading conditions be modified to mimic the variance introduced by the transition between two connected columns. Using a standard chromatography system, we evaluated a flow-through anion exchange chromatography step in a monoclonal antibody (mAb) manufacturing process using five different methods to introduce the virus to the column. Our data show that whether the virus or the mAbs were introduced in concentrated peaks, or as a homogeneous batch, the clearance of mouse minute virus was similar. This study introduces an alternative way to evaluate viral clearance in a continuous process and demonstrates the robustness of anion exchange chromatography unit operating in continuous processing.<br /> (© 2020 Wiley Periodicals LLC.)

Details

Language :
English
ISSN :
1097-0290
Volume :
117
Issue :
11
Database :
MEDLINE
Journal :
Biotechnology and bioengineering
Publication Type :
Academic Journal
Accession number :
32667685
Full Text :
https://doi.org/10.1002/bit.27500