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Molecular epidemiology and antimicrobial resistance of group a streptococcus recovered from patients in Beijing, China.
- Source :
-
BMC infectious diseases [BMC Infect Dis] 2020 Jul 13; Vol. 20 (1), pp. 507. Date of Electronic Publication: 2020 Jul 13. - Publication Year :
- 2020
-
Abstract
- Background: Group A streptococcus (GAS) is an important human pathogen responsible for a broad range of infections. Epidemiological surveillance has been crucial to detect changes in the geographical and temporal variation of the disease pattern. The objective of this study was to investigate the molecular epidemiological characteristics and antimicrobial resistance of GAS isolates from patients in Children's Hospital in Beijing.<br />Methods: From 2016 to 2017, pharyngeal swab samples were collected from the outpatients in Children's Hospital, Capital Institute of Pediatrics, who were diagnosed with scarlet fever. Antimicrobial susceptibility test was performed according to the distribution of conventional antibiotics and Clinical and Laboratory Standards Institute (CLSI) recommendations. The distribution of the macrolide-resistance genes (ermB, ermA, mefA), emm (M protein-coding gene) typing, and superantigens (SAg) gene profiling were examined by polymerase chain reaction (PCR).<br />Results: A total of 297 GAS isolates were collected. The susceptibility of the isolates to penicillin, ceftriaxone, and levofloxacin was 100%. The resistance rate to erythromycin and clindamycin was 98.3 and 96.6%, respectively. The dominant emm types were emm12 (65.32%), emm1 (27.61%), emm75 (2.69%), and emm89 (1.35%). Of the 297 isolates, 290 (97.64%) carried the ermB gene, and 5 (1.68%) carried the mefA gene, while none carried the ermA gene. The most common superantigen genes identified from GAS isolates were smeZ (96.97%), speC (92.59%), speG (91.58%), ssa (85.52%), speI (54.55%), speH (52.19%), and speA (34.34%). Isolates with the genotype emm1 possessed speA, speC, speG, speJ, speM, ssa, and smeZ, while emm12 possessed speC, speG, speH, speI, speM, ssa, and smeZ superantigens.<br />Conclusions: The prevalent strain of GAS isolates in Beijing has a high resistance rate to macrolides; however, penicillin can still be the preferred antibiotic for treatment. Erythromycin resistance was predominantly mediated by ermB. The common emm types were emm12 and emm1. There was a correlation between emm and the superantigen gene. Thus, long-term monitoring and investigation of the emm types and superantigen genes of GAS prevalence are imperative.
- Subjects :
- Adolescent
Antigens, Bacterial genetics
Bacterial Outer Membrane Proteins genetics
Bacterial Proteins genetics
Beijing epidemiology
Cardiopulmonary Resuscitation
Carrier Proteins genetics
Child
Child, Preschool
Erythromycin therapeutic use
Female
Hospitals, Pediatric
Humans
Infant
Male
Microbial Sensitivity Tests
Scarlet Fever microbiology
Streptococcus pyogenes isolation & purification
Superantigens genetics
Anti-Bacterial Agents therapeutic use
Drug Resistance, Bacterial genetics
Penicillins therapeutic use
Scarlet Fever drug therapy
Scarlet Fever epidemiology
Streptococcus pyogenes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2334
- Volume :
- 20
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 32660436
- Full Text :
- https://doi.org/10.1186/s12879-020-05241-x