Back to Search
Start Over
Effects of SFRP4 overexpression on the production of adipokines in transgenic mice.
- Source :
-
Adipocyte [Adipocyte] 2020 Dec; Vol. 9 (1), pp. 374-383. - Publication Year :
- 2020
-
Abstract
- Secreted frizzled-related protein (SFRP) 4 is an extracellular antagonist of Wnt signalling that regulates adipogenesis, and is highly in the visceral adipose tissue of obese individuals. However, it is still unclear how exactly SFRP4 regulates the secretion of adipokines in the adipose tissue in vivo , an event that is closely related to the pathogenesis of obesity and insulin resistance. In this study, we generated transgenic (Tg) mice overexpressing SFRP4 in the liver and investigated SFRP4 role in adipokine secretion in mice on a regular normal diet. In Tg mice, SFRP4 protein was overexpressed in the liver, as compared to wild-type littermates (non-Tg), and released into the blood. Moreover, the size of adipocytes was smaller in the visceral adipose tissue of Tg mice compared to controls. Additionally, SFRP4 overexpression affected the expression of genes related to adipocyte differentiation, causing the upregulation of adiponectin and glucose transporter 4, and the downregulation of CCAAT/enhancer-binding protein-β, in both visceral and subcutaneous adipose tissue. However, there was no difference in body weight or body composition between Tg and non-Tg mice. In summary, our data showed that SFRP4 overexpression altered adipocyte size and adipokine secretion, possibly affecting adipocyte differentiation, obesity, and glucose metabolism.
- Subjects :
- Adipocytes metabolism
Adipocytes pathology
Adipose Tissue, White metabolism
Animals
Cell Differentiation
Cell Size
Gene Targeting
Humans
Intra-Abdominal Fat metabolism
Male
Mice
Mice, Transgenic
Proto-Oncogene Proteins metabolism
Adipokines biosynthesis
Gene Expression
Proto-Oncogene Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2162-397X
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Adipocyte
- Publication Type :
- Academic Journal
- Accession number :
- 32657640
- Full Text :
- https://doi.org/10.1080/21623945.2020.1792614