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Quantification of SARS-CoV-2 antibodies with eight commercially available immunoassays.
- Source :
-
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2020 Aug; Vol. 129, pp. 104540. Date of Electronic Publication: 2020 Jul 06. - Publication Year :
- 2020
-
Abstract
- Since the emergence of SARS-CoV-2 numerous antibody assays have become available, demonstrating different performance characteristics. This study focused on a quantitative correlation between different commercial assays and a neutralization test (NT). Comparative data is needed as a basis for the production of convalescent plasma and potential interpretations COVID-19 immunity. Sera of 100 SARS-CoV-2 convalescent plasma donors were collected and SARS-CoV-2 antibodies were characterized using three different IgG-ELISAs (EUROIMMUN IgG and NCP-IgG ELISA, Wantai ELISA), two CLIA (Elecsys, LIAISON) and two lateral flow tests (MEDsan IgM/IgG-Rapid-Test, Wantai Rapid Test) and subsequently correlated to neutralization titers. The Wantai ELISA and the Elecsys provide the highest sensitivities in this sample (98 and 95 percent respectively). Titers with the best overall quantitative correlation to the NT titer were obtained with the Euroimmun IgG ELISA assay (Rho=0.759) and the Wantai ELISA assay (Rho=0.729). An infection without fever and negative or weakly positive reactions in the Wantai Rapid test were negative predictive factors for NT titers >1:200 (negative predictive value of 92 % and 92 % respectively, combination of both 100 %). The Wantai ELISA titer could be a suitable substitute for NT. An adequate pooling strategy of plasma units additionally could compensate deviations of individual antibody titers.<br />Competing Interests: Declaration of Competing Interest MRF and TRK are employees of Baxter AG, a Takeda company and have Takeda stock interest. All other authors declare no conflict of interests.<br /> (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Adolescent
Adult
Aged
Antibodies, Neutralizing blood
COVID-19
COVID-19 Testing
Female
Humans
Immunoglobulin G blood
Male
Middle Aged
Pandemics
Predictive Value of Tests
SARS-CoV-2
Sensitivity and Specificity
Young Adult
Antibodies, Viral blood
Betacoronavirus immunology
Clinical Laboratory Techniques methods
Coronavirus Infections diagnosis
Pneumonia, Viral diagnosis
Serologic Tests methods
Subjects
Details
- Language :
- English
- ISSN :
- 1873-5967
- Volume :
- 129
- Database :
- MEDLINE
- Journal :
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
- Publication Type :
- Academic Journal
- Accession number :
- 32652475
- Full Text :
- https://doi.org/10.1016/j.jcv.2020.104540