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Metagenome Data on Intestinal Phage-Bacteria Associations Aids the Development of Phage Therapy against Pathobionts.
- Source :
-
Cell host & microbe [Cell Host Microbe] 2020 Sep 09; Vol. 28 (3), pp. 380-389.e9. Date of Electronic Publication: 2020 Jul 10. - Publication Year :
- 2020
-
Abstract
- The application of bacteriophages (phages) is proposed as a highly specific therapy for intestinal pathobiont elimination. However, the infectious associations between phages and bacteria in the human intestine, which is essential information for the development of phage therapies, have yet to be fully elucidated. Here, we report the intestinal viral microbiomes (viromes), together with bacterial microbiomes (bacteriomes), in 101 healthy Japanese individuals. Based on the genomic sequences of bacteriomes and viromes from the same fecal samples, the host bacteria-phage associations are illustrated for both temperate and virulent phages. To verify the usefulness of the comprehensive host bacteria-phage information, we screened Clostridioides difficile-specific phages and identified antibacterial enzymes whose activity is confirmed both in vitro and in vivo. These comprehensive metagenome analyses reveal not only host bacteria-phage associations in the human intestine but also provide vital information for the development of phage therapies against intestinal pathobionts.<br />Competing Interests: Declaration of Interests The authors declare no competing interests.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Anti-Bacterial Agents pharmacology
Bacteriophages isolation & purification
Clostridium Infections therapy
Disease Models, Animal
Endopeptidases pharmacology
Feces microbiology
Female
Genome, Bacterial
Genome, Viral
Humans
Metagenome
Mice
Mice, Inbred C57BL
Sequence Analysis, DNA
Specific Pathogen-Free Organisms
Viral Proteins genetics
Viral Proteins pharmacology
Bacteriophages genetics
Clostridioides difficile virology
Endopeptidases genetics
Gastrointestinal Microbiome genetics
Phage Therapy methods
Prophages genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1934-6069
- Volume :
- 28
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cell host & microbe
- Publication Type :
- Academic Journal
- Accession number :
- 32652061
- Full Text :
- https://doi.org/10.1016/j.chom.2020.06.005