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Nanosensors based on LSPR are able to serologically differentiate dengue from Zika infections.
- Source :
-
Scientific reports [Sci Rep] 2020 Jul 09; Vol. 10 (1), pp. 11302. Date of Electronic Publication: 2020 Jul 09. - Publication Year :
- 2020
-
Abstract
- The Flaviviridae virus family was named after the Yellow-fever virus, and the latin term flavi means "of golden color". Dengue, caused by Dengue virus (DENV), is one of the most important infectious diseases worldwide. A sensitive and differential diagnosis is crucial for patient management, especially due to the occurrence of serological cross-reactivity to other co-circulating flaviviruses. This became particularly important with the emergence of Zika virus (ZIKV) in areas were DENV seroprevalence was already high. We developed a sensitive and specific diagnostic test based on gold nanorods (GNR) functionalized with DENV proteins as nanosensors. These were able to detect as little as one picogram of anti-DENV monoclonal antibodies and highly diluted DENV-positive human sera. The nanosensors could differentiate DENV-positive sera from other flavivirus-infected patients, including ZIKV, and were even able to distinguish which DENV serotype infected individual patients. Readouts are obtained in ELISA-plate spectrophotometers without the need of specific devices.
- Subjects :
- Antibodies, Viral blood
Brazil
Cohort Studies
Dengue virology
Gold chemistry
Humans
Metal Nanoparticles chemistry
Seroepidemiologic Studies
Viral Envelope Proteins immunology
Zika Virus Infection virology
Biosensing Techniques methods
Dengue diagnosis
Surface Plasmon Resonance methods
Zika Virus Infection diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 32647259
- Full Text :
- https://doi.org/10.1038/s41598-020-68357-9