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Injury Length and Arteriole Constriction Shape Clot Growth and Blood-Flow Acceleration in a Mouse Model of Thrombosis.
- Source :
-
Arteriosclerosis, thrombosis, and vascular biology [Arterioscler Thromb Vasc Biol] 2020 Sep; Vol. 40 (9), pp. 2114-2126. Date of Electronic Publication: 2020 Jul 09. - Publication Year :
- 2020
-
Abstract
- Objective: Quantitative relationships between the extent of injury and thrombus formation in vivo are not well understood. Moreover, it has not been investigated how increased injury severity translates to blood-flow modulation. Here, we investigated interconnections between injury length, clot growth, and blood flow in a mouse model of laser-induced thrombosis. Approach and Results: Using intravital microscopy, we analyzed 59 clotting events collected from the cremaster arteriole of 14 adult mice. We regarded injury length as a measure of injury severity. The injury caused transient constriction upstream and downstream of the injury site resulting in a 50% reduction in arteriole diameter. The amount of platelet accumulation and fibrin formation did not depend on arteriole diameter or deformation but displayed an exponentially increasing dependence on injury length. The height of the platelet clot depended linearly on injury length and the arteriole diameter. Upstream arteriolar constriction correlated with delayed upstream velocity increase, which, in turn, determined downstream velocity. Before clot formation, flow velocity positively correlated with the arteriole diameter. After the onset of thrombus growth, flow velocity at the injury site negatively correlated with the arteriole diameter and with the size of the above-clot lumen.<br />Conclusions: Injury severity increased platelet accumulation and fibrin formation in a persistently steep fashion and, together with arteriole diameter, defined clot height. Arterial constriction and clot formation were characterized by a dynamic change in the blood flow, associated with increased flow velocity.
- Subjects :
- Animals
Arterioles injuries
Arterioles physiopathology
Blood Flow Velocity
Blood Platelets metabolism
Constriction, Pathologic
Disease Models, Animal
Fibrin metabolism
Intravital Microscopy
Male
Mice
Microscopy, Fluorescence
Severity of Illness Index
Thrombosis blood
Thrombosis physiopathology
Time Factors
Vascular System Injuries blood
Vascular System Injuries physiopathology
Abdominal Muscles blood supply
Arterioles pathology
Blood Coagulation
Thrombosis pathology
Vascular System Injuries pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1524-4636
- Volume :
- 40
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Arteriosclerosis, thrombosis, and vascular biology
- Publication Type :
- Academic Journal
- Accession number :
- 32640902
- Full Text :
- https://doi.org/10.1161/ATVBAHA.120.314786