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Cytotoxicity of Saikosaponin A targets HEKa cell through apoptosis induction by ROS accumulation and inflammation suppression via NF-κB pathway.
- Source :
-
International immunopharmacology [Int Immunopharmacol] 2020 Sep; Vol. 86, pp. 106751. Date of Electronic Publication: 2020 Jul 04. - Publication Year :
- 2020
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Abstract
- Saikosaponin A (SSA) is a triterpenoid saponin extracted from oriental medicinal plant Radix bupleuri, possessing various biological functions such as anti-inflammatory, immune regulation and anti-virus. This study aimed to explore therapeutic effects of SSA on psoriasis in both vitro and vivo. Our results showed that SSA increased reactive oxygen species (ROS) generation, and decreased mitochondrial membrane potential (MMP) and M5-induced inflammatory cytokines levels in HEKa cells in a dose-dependent manner. In addition, SSA promoted apoptosis and suppressed phosphorylation of NF-κB in vitro, which were restored by the ROS scavenger N-acetylcysteine (NAC). In imiquimod (IMQ)-induced mice, gavage with SSA markedly decreased Psoriasis Area and Severity Index (PASI) score and ameliorated epidermal hyperplasia through inhibition of NF-κB and NLRP3 signaling pathway. In conclusion, our studies demonstrate that SSA induces apoptosis and suppresses inflammation in HEKa cells and ameliorates IMQ-induced psoriasis in mice, making it a therapeutic candidate for psoriasis.<br />Competing Interests: Declaration of Competing Interests The authors declare that there is no conflict of interests.<br /> (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Acetylcysteine metabolism
Animals
Apoptosis
Cell Line
Humans
Imiquimod metabolism
Immunosuppression Therapy
Mice
Oleanolic Acid metabolism
Reactive Oxygen Species metabolism
Signal Transduction
Anti-Inflammatory Agents, Non-Steroidal pharmacology
Inflammation drug therapy
Keratinocytes immunology
NF-kappa B metabolism
Oleanolic Acid analogs & derivatives
Saponins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1705
- Volume :
- 86
- Database :
- MEDLINE
- Journal :
- International immunopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 32634696
- Full Text :
- https://doi.org/10.1016/j.intimp.2020.106751