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Lipid composition and abundance in the reproductive and alimentary tracts of female Haemonchus contortus.
- Source :
-
Parasites & vectors [Parasit Vectors] 2020 Jul 06; Vol. 13 (1), pp. 338. Date of Electronic Publication: 2020 Jul 06. - Publication Year :
- 2020
-
Abstract
- Background: Lipids play essential structural and functional roles in the biology of animals. Studying the composition and abundance of lipids in parasites should assist in gaining a better understanding of their molecular biology, biochemistry and host-parasite interactions.<br />Methods: Here, we used a combination of high-performance liquid chromatography and mass spectrometric analyses, combined with bioinformatics, to explore the lipid composition and abundance in the reproductive (Rt) and alimentary (At) tracts of Haemonchus contortus.<br />Results: We identified and quantified 320 unique lipid species representing four categories: glycerolipids, glycerophospholipids, sphingolipids and steroid lipids. Glycerolipids (i.e. triradylglycerols) and glycerophospholipids (i.e. glycerophosphocholines) were the most commonly and abundant lipid classes identified and were significantly enriched in Rt and At, respectively. We propose that select parasite-derived lipids in Rt and At of adult female H. contortus are required as an energy source (i.e. triradylglycerol) or are involved in phospholipid biosynthesis (i.e. incorporated fatty acids) and host-parasite interactions (i.e. phospholipids and lysophospholipids).<br />Conclusions: This work provides a first foundation to explore lipids at the organ-specific and tissue-specific levels in nematodes, and to start to unravel aspects of lipid transport, synthesis and metabolism, with a perspective on discovering new intervention targets.
- Subjects :
- Animals
Chromatography, Liquid methods
Digestive System metabolism
Female
Genitalia metabolism
Haemonchiasis parasitology
Host-Parasite Interactions
Life Cycle Stages physiology
Mass Spectrometry methods
Nematoda
Sex Factors
Haemonchus metabolism
Lipid Metabolism
Lipids analysis
Lipids chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1756-3305
- Volume :
- 13
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Parasites & vectors
- Publication Type :
- Academic Journal
- Accession number :
- 32631412
- Full Text :
- https://doi.org/10.1186/s13071-020-04208-w