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Distinct Local and Systemic Molecular Signatures in the Esophageal and Gastric Cancers: Possible Therapy Targets and Biomarkers for Gastric Cancer.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2020 Jun 25; Vol. 21 (12). Date of Electronic Publication: 2020 Jun 25. - Publication Year :
- 2020
-
Abstract
- Gastric (GC) and esophageal (EC) cancers are highly lethal. Better understanding of molecular abnormalities is needed for new therapeutic targets and biomarkers to be found. Expression of 18 cancer-related genes in 31 paired normal-tumor samples was quantified by reversely-transcribed quantitative polymerase chain reaction (RTqPCR) and systemic concentration of 27 cytokines/chemokines/growth factors in 195 individuals was determined using Luminex xMAP technology. Only Ki67 , CLDN2, and BCLxL were altered in GC while Ki67 , CDKN1A , ODC1 , SLC2A1 , HIF1A , VEGFA , NOS2 , CCL2 , PTGS2 , IL10 , IL10Ra, and ACTA2 were changed in EC. The relatively unaltered molecular GC landscape resulted from high expression of BCLxL , CDKN1A , BCL2 , Ki67 , HIF1A , VEGFA , ACTA2 , TJP1 , CLDN2 , IL7Ra , ODC1 , PTGS2 , and CCL2 in non-cancerous tissue. The NOS2 expression and IL-4, IL-9, FGF2, and RANTES secretion were higher in cardiac than non-cardiac GC. Four-cytokine panels (interleukin (IL)-1β/IL-1ra/IL-6/RANTES or IL-1β/IL-6/IL-4/IL-13) differentiated GC from benign conditions with 87-89% accuracy. Our results showed increased proliferative, survival, inflammatory and angiogenic capacity in gastric tumor-surrounding tissue, what might contribute to GC aggressiveness and facilitate cancer recurrence. Further studies are needed to determine the CLDN2 and NOS2 suitability as candidate molecular targets in GC and cardiac GC, respectively, and discern the role of CLDN2 or to verify IL-1β/IL-1ra/IL-6/RANTES or IL-1β/IL-6/IL-4/IL-13 usefulness as differential biomarkers.
- Subjects :
- Aged
Biomarkers, Tumor genetics
Cell Line, Tumor
Chemokine CCL5 genetics
Cytokines genetics
Esophageal Neoplasms metabolism
Female
Gene Expression genetics
Gene Expression Profiling methods
Humans
Interleukin-1beta genetics
Interleukin-6 genetics
Male
Middle Aged
Neoplasm Recurrence, Local genetics
Stomach Neoplasms metabolism
Stomach Neoplasms pathology
Transcriptome genetics
Esophageal Neoplasms genetics
Stomach Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 21
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 32630408
- Full Text :
- https://doi.org/10.3390/ijms21124509