IDP-LZerD: Software for Modeling Disordered Protein Interactions.

Modeling the tertiary structure of protein-protein interaction complex has been well studied over many years, especially in the case where the structures of both binding partners are roughly the same before and after binding. However, the assembly of complexes with less-ordered partners is a much harder problem, and modeling even small amounts of flexibility can pose a challenge. In an extreme case, where one of the binding partners is intrinsically disordered before binding, we have previously shown that by initially disregarding the coupling between windows of these intrinsically disordered proteins (IDPs), we can reliably assemble complexes involving IDPs up to at least 69 residues long. Here, we detail the use of the IDP-LZerD package and protocol.