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The temperature dependence of kinesin motor-protein mechanochemistry.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2020 Aug 27; Vol. 529 (3), pp. 812-818. Date of Electronic Publication: 2020 Jun 30. - Publication Year :
- 2020
-
Abstract
- Biophysical studies of the mechanochemical cycle of kinesin motors are essential for understanding the mechanism of energy conversion. Here, we report a systematic study of the impact of temperature on velocity and run length of homodimeric Drosophila kinesin-1, homodimeric C. elegans OSM-3 and heterodimeric C. elegans kinesin-II motor proteins using in vitro single-molecule motility assays. Under saturated ATP conditions, kinesin-1 and OSM-3 are fast and processive motors compared to kinesin-II. From in vitro motility assays employing single-molecule fluorescence microscopy, we extracted single-motor velocities and run lengths in a temperature range from 15 °C to 35 °C. Both parameters showed a non-Arrhenius temperature dependence for all three motors, which could be quantitatively modeled using a simplified, two-state kinetic model of the mechanochemistry of the three motors, providing new insights in the temperature dependence of their mechanochemistry.<br /> (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Biomechanical Phenomena
Caenorhabditis elegans chemistry
Caenorhabditis elegans Proteins chemistry
Drosophila Proteins chemistry
Drosophila melanogaster chemistry
Kinesins chemistry
Models, Biological
Motion
Protein Multimerization
Temperature
Caenorhabditis elegans metabolism
Caenorhabditis elegans Proteins metabolism
Drosophila Proteins metabolism
Drosophila melanogaster metabolism
Kinesins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 529
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 32620235
- Full Text :
- https://doi.org/10.1016/j.bbrc.2020.06.004