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A Whole-Plant Screening Test to Select Freezing-Tolerant and Low-Dormant Genotypes.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2156, pp. 53-60. - Publication Year :
- 2020
-
Abstract
- Winter survival is a determinant factor for the persistence of perennials grown in northern climates. High winter survival cultivars, however, have lower yield due to their early transition into a dormant state in the fall. Here we describe a whole plant assay entirely performed indoor in growth chambers and walk-in freezers to identify low-dormant genotypes with superior freezing tolerance within populations of open pollinated species. Three successive freezing stresses are applied to a broad base of 3000 genotypes to progressively eliminate 97% of the population and to retain only the 3% best performing genotypes. This approach can be used to generate recurrently selected populations in different species.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2156
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 32607975
- Full Text :
- https://doi.org/10.1007/978-1-0716-0660-5_6