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In vivo stem cell tracking using scintigraphy in a canine model of DMD.
- Source :
-
Scientific reports [Sci Rep] 2020 Jun 30; Vol. 10 (1), pp. 10681. Date of Electronic Publication: 2020 Jun 30. - Publication Year :
- 2020
-
Abstract
- One of the main challenges in cell therapy for muscle diseases is to efficiently target the muscle. To address this issue and achieve better understanding of in vivo cell fate, we evaluated the relevance of a non-invasive cell tracking method in the Golden Retriever Muscular Dystrophy (GRMD) model, a well-recognised model of Duchenne Muscular Dystrophy (DMD). Mesoangioblasts were directly labelled with <superscript>111</superscript> In-oxine, and injected through one of the femoral arteries. The scintigraphy images obtained provided the first quantitative mapping of the immediate biodistribution of mesoangioblasts in a large animal model of DMD. The results revealed that cells were trapped by the first capillary filters: the injected limb and the lung. During the days following injection, radioactivity was redistributed to the liver. In vitro studies, performed with the same cells prepared for injecting the animal, revealed prominent cell death and <superscript>111</superscript> In release. In vivo, cell death resulted in <superscript>111</superscript> In release into the vasculature that was taken up by the liver, resulting in a non-specific and non-cell-bound radioactive signal. Indirect labelling methods would be an attractive alternative to track cells on the mid- and long-term.
- Subjects :
- Animals
Cell Differentiation physiology
Cell Tracking methods
Disease Models, Animal
Dogs
Dystrophin metabolism
Female
Male
Muscle, Skeletal metabolism
Muscle, Skeletal pathology
Muscular Dystrophy, Animal metabolism
Muscular Dystrophy, Duchenne metabolism
Radionuclide Imaging methods
Stem Cells metabolism
Tissue Distribution physiology
Cell Movement physiology
Muscular Dystrophy, Animal pathology
Muscular Dystrophy, Duchenne pathology
Stem Cells pathology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 32606364
- Full Text :
- https://doi.org/10.1038/s41598-020-66388-w