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Determination of testosterone/epitestosterone concentration ratio in human urine by capillary electrophoresis.
- Source :
-
Steroids [Steroids] 2020 Sep; Vol. 161, pp. 108691. Date of Electronic Publication: 2020 Jun 27. - Publication Year :
- 2020
-
Abstract
- A novel method for determining the testosterone/epitestosterone concentration ratio in human urine was established by capillary electrophoresis with diode-array detector. The urine samples were firstly purified by the solid extraction. The optimal experimental conditions were: running buffer pH = 4.74, 15.0 mmol L <superscript>-1</superscript> HAc-NaAc, separation voltage 25 kV, temperature 25 °C, sample injection pressure 3.43 × 10 <superscript>3</superscript> Pa, and duration 10 s. The testosterone and epitestosterone linear range were determined as 8.0-960.0 ng mL <superscript>-1</superscript> , respectively. The testosterone and epitestosterone detection limits were determined as 4.6 and 4.5 ng mL <superscript>-1</superscript> , respectively. The relative standard deviation was less than 0.36%.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1878-5867
- Volume :
- 161
- Database :
- MEDLINE
- Journal :
- Steroids
- Publication Type :
- Academic Journal
- Accession number :
- 32603755
- Full Text :
- https://doi.org/10.1016/j.steroids.2020.108691