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Fluorescence Enhancement Effect by Metal Nanoparticles-immobilized Microplate.
- Source :
-
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2020 Jul 10; Vol. 36 (7), pp. 787-789. Date of Electronic Publication: 2020 Jun 26. - Publication Year :
- 2020
-
Abstract
- In this reported work, we achieved high-throughput, highly sensitive fluorescent analysis using an enzyme-linked immunosorbent assay (ELISA) that employed a metallic nanoparticle (NP)-immobilized 96-well plate. The immobilization of metallic NPs on a 96-well plate effectively amplified fluorescent signals of the assay. The silver (Ag) NP-immobilized plate showed the best fluorescent enhancement effect of all plates immobilized by metal NPs. Our results demonstrate the potential of applying Ag NPs to enhance the efficiency of direct and indirect ELISA by the labeling of targets.
Details
- Language :
- English
- ISSN :
- 1348-2246
- Volume :
- 36
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32595192
- Full Text :
- https://doi.org/10.2116/analsci.advpub_20C009