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Microfluidic Paper-Based Analytical Device for Histidine Determination.
- Source :
-
Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2020 Nov; Vol. 192 (3), pp. 812-821. Date of Electronic Publication: 2020 Jun 27. - Publication Year :
- 2020
-
Abstract
- A laminated paper-based analytical device (LPAD) for histidine detection was fabricated from a chromatography filtration paper and laminate films. Histidine recognition was effected by histidyl-tRNA synthetase (HisRS), and its detection was signaled colorimetrically based on the molybdenum blue reaction. The analytical conditions and detectable concentration range of histidine were examined. The method provided selective quantification from 1 to 100 μM histidine. LPAD fabrication is considerably simple, involving only the craft-cutting of the chromatography filtration paper and laminate film, and is cost-effective.
Details
- Language :
- English
- ISSN :
- 1559-0291
- Volume :
- 192
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Applied biochemistry and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 32592084
- Full Text :
- https://doi.org/10.1007/s12010-020-03365-z