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An Artificial Hemoprotein with Inducible Peroxidase- and Monooxygenase-Like Activities.
- Source :
-
Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2020 Nov 20; Vol. 26 (65), pp. 14929-14937. Date of Electronic Publication: 2020 Oct 16. - Publication Year :
- 2020
-
Abstract
- A novel inducible artificial metalloenzyme obtained by covalent attachment of a manganese(III)-tetraphenylporphyrin (MnTPP) to the artificial bidomain repeat protein, (A3A3')Y26C, is reported. The protein is part of the αRep family. The biohybrid was fully characterized by MALDI-ToF mass spectrometry, circular dichroism and UV/Vis spectroscopies. The peroxidase and monooxygenase activities were evaluated on the original and modified scaffolds including those that have a) an additional imidazole, b) a specific αRep bA3-2 that is known to induce the opening of the (A3A3') interdomain region and c) a derivative of the αRep bA3-2 inducer extended with a His <subscript>6</subscript> -Tag (His <subscript>6</subscript> -bA3-2). Catalytic profiles are highly dependent on the presence of co-catalysts with the best activity obtained with His <subscript>6</subscript> -bA3-2. The entire mechanism was rationalized by an integrative molecular modeling study that includes protein-ligand docking and large-scale molecular dynamics. This constitutes the first example of an entirely artificial metalloenzyme with inducible peroxidase and monooxygenase activities, reminiscent of allosteric regulation of natural enzymatic pathways.<br /> (© 2020 Wiley-VCH GmbH.)
- Subjects :
- Catalysis
Metalloproteins
Peroxidases
Mixed Function Oxygenases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1521-3765
- Volume :
- 26
- Issue :
- 65
- Database :
- MEDLINE
- Journal :
- Chemistry (Weinheim an der Bergstrasse, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 32588931
- Full Text :
- https://doi.org/10.1002/chem.202002434